A Semi-Synthetic Human Hyperimmune Antibody Library for Drug Discovery

Here we describe the construction of a semi-synthetic phage display human antibody library derived from mining a public database of human naive and memory B-cell receptor sequences consisting of more than 37 million unique lgH sequences from each of 3 healthy donors. More than 2 million HCDR3 sequences were gathered from the analysis and individually constructed with Twist’s unique silicon-based DNA synthesis capabilities.