Diversify your antibody discovery
Diversify your antibody discovery
Diversify your antibody discovery
OVERVIEW SPECIFICATIONS PROOF OF CONCEPT RESOURCES
OVERVIEW

Explore VHH sequence space with three new single domain antibody libraries.

VHH Ratio

Specific oligo pools model the natural VHH repertoire

VHH Shuffle

Natural llama CDR sequences in the context of a llama consensus framework

VHH hShuffle

Natural llama CDR sequences in the context of a partially humanized VHH framework

Icon benefit fast pink
No Hassle
Stable & robust
Easier to engineer & manufacture
Faster than traditional approaches – no immunization required
Icon benefit modular pink
Intelligent Design
Small & modular antibodies
Create building blocks for bispecific antibodies
Icon Benefit scale pink
Discovery without constraints
Full antigen binding capacity
Small size allows access to epitopes usually sterically hindered by an IgG

Explore VHH sequence space with three new single domain antibody libraries.

VHH Ratio

Specific oligo pools model the natural VHH repertoire

VHH Shuffle

Natural llama CDR sequences in the context of a llama consensus framework

VHH hShuffle

Natural llama CDR sequences in the context of a partially humanized VHH framework

Icon benefit fast pink
No Hassle
Stable & robust
Easier to engineer & manufacture
Faster than traditional approaches – no immunization required
Icon benefit modular pink
Intelligent Design
Small & modular antibodies
Create building blocks for bispecific antibodies
Icon Benefit scale pink
Discovery without constraints
Full antigen binding capacity
Small size allows access to epitopes usually sterically hindered by an IgG
SPECIFICATIONS
VHH Ratio
  • 2391 CDR sequences analyzed for position-specific variation
  • Controlled CDR diversity introduced in the library
  • Consensus llama framework
VHH Schematic
VHH Shuffle
  • Each unique CDR individually synthesized
  • Shuffled in consensus llama framework
  • Final diversity of library > theoretical diversity
  • Theoretical library diversity of 3.2 × 10^9
VHH Schematic 2
VHH hShuffle
  • Shuffled CDRs with theoretical library diversity of 3.2 × 109
  • Partially humanized framework: Framework 1, 3 and 4 were 
    humanized using the human germline DP-47 framework
VHH Schematic 3
VHH Ratio
  • 2391 CDR sequences analyzed for position-specific variation
  • Controlled CDR diversity introduced in the library
  • Consensus llama framework
VHH Schematic
VHH Shuffle
  • Each unique CDR individually synthesized
  • Shuffled in consensus llama framework
  • Final diversity of library > theoretical diversity
  • Theoretical library diversity of 3.2 × 10^9
VHH Schematic 2
VHH hShuffle
  • Shuffled CDRs with theoretical library diversity of 3.2 × 109
  • Partially humanized framework: Framework 1, 3 and 4 were 
    humanized using the human germline DP-47 framework
VHH Schematic 3
PROOF OF CONCEPT
ELISA+ colony count

1 × 384 well plate picked per library per round

VHH Table
Array SPR Binding Analysis

Out of 140 VHH binders

  • 51 variants < 100 nM
  • 90 variants < 200 nM
VHH AffinityDist
CDRH3 length distributions show

Anti-TIGIT clones from VHH libraries encompass a range of affinities and diversity

VHH CDR3Counts
ELISA+ colony count

1 × 384 well plate picked per library per round

VHH Table
Array SPR Binding Analysis

Out of 140 VHH binders

  • 51 variants < 100 nM
  • 90 variants < 200 nM
VHH AffinityDist
CDRH3 length distributions show

Anti-TIGIT clones from VHH libraries encompass a range of affinities and diversity

VHH CDR3Counts
RESOURCES